产品详情
产品名称MDM2(phospho-Ser166) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human
特异性The antibody detects endogenous level of MDM2 only when phosphorylated at serine 166.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of Serine 166 (A-I-S(p)-E-T) derived from Human MDM2.
基因/蛋白名称MDM2
标记Unconjugated
别名HDMX, hdm2
数据库入口号Swiss-Prot: Q00987
NCBI Protein: NP_002383.2
Uniprot
Q00987
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 90kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells untreated or treated with Hydroxyurea using MDM2(phospho-Ser166) Antibody #11550.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2(Phospho-Ser166) Antibody #11550(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using MDM2(phospho-Ser166) Antibody #11550.
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues
Haupt, Y. et al. (1997) Nature 387, 296-299.
Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.
Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.
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et al,Pathological Signaling via Platelet-Derived Growth Factor Receptor Involves Chronic Activation of Akt and Suppression of p53
, (2011),
PMID:
21357737
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