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PDHK1(Phospho-Thr338) Antibody#11596

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产品详情

产品名称PDHK1(Phospho-Thr338) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Human;Mouse;Rat

特异性The antibody detects endogenous level of PDHK1 only when phosphorylated at threonine 338.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 338(Y-S-T(p)-A-P) derived from Human PDHK1.

基因/蛋白名称PDHK1

标记Unconjugated

别名PDK1; PDH kinase 1

数据库入口号Swiss-Prot: Q15118
NCBI Protein: NP_001265478.1

Uniprot Q15118

计算分子量49kd

浓度1.0mg/mL

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Target种属Human

应用详情

 Western blotting: 1:500~1:1000

Immunoblotting analysis of PDHK1 and PDH phosphorylation in mitochondria. Mitochondrial fractions were prepared and immunoblotted with indicated antibodies
 Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
 
Gudi R., Bowker-Kinley M.M., Kedishvili N.Y., Zhao Y., Popov K.M.J. Biol. Chem. 270:28989-28994(1995)            
The MGC Project TeamGenome Res. 14:2121-2127(2004)
 

 

如果您使用该产品11596发表了文章,请通知我们,让我们可以引用您的文献。

et al,Mitochondria-Translocated PGK1 Functions as a Protein Kinase to Coordinate Glycolysis and the TCA Cycle in Tumorigenesis. , (2016 Mar),
PMID: 26942675
et al,Associations of PGK1 promoter hypomethylation and PGK1-mediated PDHK1 phosphorylation with cancer stage and prognosis: a TCGA pan-cancer analysis. In Cancer Commun (Lond) on 2019 Oct 2 by Shao F, Yang X,et al..PMID:31578148 , (2019),
PMID: 31578148
et al,O-GlcNAcylation of PGK1 coordinates glycolysis and TCA cycle to promote tumor growth.In Nat Commun on 2020 Jan 7; by Nie H, Ju H, et al..PMID:31911580 , (2020),
PMID: 31911580
et al,Identification of a novel non-ATP-competitive protein kinase inhibitor of PGK1 from marine nature products. In Biochem Pharmacol on 2021 Jan by Yuying Wang, Lulu Sun, et al..PMID:33212041 , (2021),
PMID: 33212041
et al,Suppression of Pyruvate Dehydrogenase Kinase by Dichloroacetate in Cancer and Skeletal Muscle Cells Is Isoform Specific and Partially Independent of HIF-1伪. In Int J Mol Sci on 2021 Aug 10 by Nives 艩korja Mili膰, Klemen Dolinar, et al..PMID:34445316 , (2021),
PMID: 34445316
et al,Inhibition of phosphoglycerate kinase 1 attenuates autoimmune myocarditis by reprogramming CD4+ T cell metabolismInCardiovasc ResOn2023 Jun 13byYang Lu?1?2?3?4,?Ning Zhao et al..PMID: 36726197 , (2023),
PMID: 36726197
et al,Integrative proteogenomic characterization of early esophageal cancerInNat CommunOn 2023 Mar 25byLingling Li , Dongxian Jiang et al..PMID:36966136 , (2023),
PMID: 36966136
查看更多
et al,PRMT1-mediated PGK1 arginine methylation promotes colorectal cancer glycolysis and tumorigenesis. In Cell Death Dis on 2024 Feb 24 by Hao Liu, Xintian Chen, et al..PMID:38402202 , (2024),
PMID: 38402202
et al,Hypoxia-induced downregulation of PGK1crotonylation promotes tumorigenesis bycoordinating glycolysis and the TCA cycle. In Nat Commun on 2024 Aug 12 by Zihao Guo, Yang Zhang,et al..PMID:39134530 , (2024),
PMID: 39134530

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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