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位置: 首页 > 甲基化/乙酰化抗体 > PGK1 (Acetyl-Lys388) Antibody

PGK1 (Acetyl-Lys388) Antibody#11599

是否有货: Yes
产品详情

产品名称PGK1 (Acetyl-Lys388) Antibody

来源种属Rabbit

克隆性Polyclonal

应用WB IHC IP

种属反应性Human;Mouse

特异性The antibody detects endogenous level of PGK1 only when acetylated at Lysine 388.

免疫原类型Peptide

免疫原描述Peptide sequence around acetylation site of lysine 388(E-D-K(Acetyl)-V-S) derived from Human PGK1.

标记Unconjugated

别名MGC117307,MGC142128,MGC8947,MIG10,PGKA

数据库入口号Swiss-Prot#: P00558
NCBI Gene ID: 5230
NCBI mRNA#: NM_000291.3
NCBI Protein#: NP_000282.1

Uniprot P00558

计算分子量45KD

浓度1.0mg/mL

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C

应用详情

 WB dilution: 1:500~1:1000

 IHC dilution: 1:50~1:100

 IP dilution: 1:20~1:50

The indicated His-PGK1 proteins immobilized on Ni-NTA agarose beads was incubated with Flag-ARD1 and Ac-CoA, followed by incubation with purified GST-Beclin1 or GST-Beclin1 S30A, in the presence of [γ-32P] ATP. Autoradiography was performed.

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D).

如果您使用该产品11599发表了文章,请通知我们,让我们可以引用您的文献。

et al,Phosphoglycerate Kinase 1 Phosphorylates Beclin1 to Induce Autophagy.In Mol Cell on 2017 Mar 2 by Xu Qian , Xinjian Li,et al..PMID: 28238651 , (2017),
PMID: 28238651
et al,Identification of a novel non-ATP-competitive protein kinase inhibitor of PGK1 from marine nature products. In Biochem Pharmacol on 2021 Jan by Yuying Wang, Lulu Sun, et al..PMID:33212041 , (2021),
PMID: 33212041

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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