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位置: 首页 > 磷酸化抗体 > Arrestin 1 (Phospho-Ser412) Antibody

Arrestin 1 (Phospho-Ser412) Antibody#11654

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产品详情

产品名称Arrestin 1 (Phospho-Ser412) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Human

特异性The antibody detects endogenous levels of Arrestin-1 only when phosphorylated at serine 412.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 412 (T-G-S(p)-P-Q) derived from Human Arrestin 1.

基因/蛋白名称Arrestin 1

标记Unconjugated

别名ARR1; arrestin 2; arrestin beta 1; beta-arrestin-1;

数据库入口号Swiss-Prot#: P49407;
NCBI Gene#: 408;
NCBI Protein#: NP_004032.2.

Uniprot P49407

实际分子量50kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from COS7 cells treated with Etoposide using Arrestin 1 (Phospho-Ser412) Antibody #11654.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Arrestin 1 (Phospho-Ser412) antibody #11654 (left)or the same antibody preincubated with blocking peptide (right).

Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation.

Greg Buchanan. AACR Meeting Abstracts, Apr 2006; 2006: 430.
D. Alex Groarke. Mol. Pharmacol., Feb 2001; 59: 375.
Karen McConalogue. Mol. Biol. Cell, Aug 1998; 9: 2305.
Maria Stella Lombardi. Stroke, Apr 2004; 35: 981 - 986.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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