产品详情
产品名称CDK1/CDC2 (Phospho-Thr14) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of CDK1/CDC2 only when phosphorylated at threonine 14.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phospho rylation site of threonine14(E-G-T(p)-Y-G) derived from Human CDK1/CDC2 .
基因/蛋白名称CDK1/CDC2
标记Unconjugated
别名CDC28; CDC2A; CDK1; MPF; kinase Cdc2
数据库入口号Swiss-Prot#: P24941;
NCBI Gene#: 1017;
NCBI Protein#: NP_001277159.1
Uniprot
P24941
实际分子量34kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C/1 year
应用详情
Western blotting: 1:500~1:1000
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus.
Sarkar S, Dey BK, Dutta A (2010) Mol Biol Cell 21, 2138-49
Shreeram S, Hee WK, Bulavin DV (2008) Mol Cell Biol 28, 7442-50
Mayya V, et al. (2006) Mol Cell Proteomics 5, 1146-57
如果您使用该产品11997发表了文章，请通知我们，让我们可以引用您的文献。
et al,The Role and Mechanism of CRT0066101 as an Effective Drug for Treatment of Triple-Negative Breast Cancer. In Cell Physiol Biochem on 2019 by Liu Y, Wang Y, et al.. PMID:30845378
, (2019),
PMID:
30845378
et al,Adhesion glycoprotein CD44 functions as an upstream regulator of a network connecting ERK, AKT and Hippo-YAP pathways in cancer progression. Oncotarget on 2015 Feb 20 by Shiyi Yu , Xiuxiu Cai et al..PMID: 25605020
, (2015),
PMID:
25605020
有货
