产品详情
产品名称MITF (Phospho-Ser180/73) Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of MITF only when phosphorylated at Serine 180/73.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of Serine 180(P-N-S(p)-P-M)/Serine 73(Q-E-R(p)-R-E) derived from Human MITF.
基因/蛋白名称MITF
标记Unconjugated
别名MI, WS2, CMM8, WS2A, bHLHe32
数据库入口号Swiss-Prot#: O75030;
NCBI Gene#: 4286;
NCBI Protein#: XP_005264811.1
Uniprot
O75030
实际分子量52kd
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C/1 year
应用详情
Predicted MW: 52kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Mouse brain tissue using MITF (Phospho-Ser180/73) Antibody #12018.The lane on the right is treated with the antigen-specific peptide.
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
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et al,Dioscin Derived From Solanum Melongena L. "Usukawamarunasu" Attenuates 伪-MSH-Induced Melanogenesis in B16 Murine Melanoma Cells via Downregulation of Phospho-CREB and MITF .In J Food Sci on 2015 Oct by Atsuyoshi Nishina, Kodai Ebina et al..PMID:26352003
, (2015),
PMID:
26352003
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