产品详情
产品名称BCL-2 (Phospho-Thr69) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human
特异性The antibody detects endogenous level of BCL-2 only when phosphorylated at Threonine 69.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of Threonine 69
(A-R-T(p)-S-P) derived from Human BCL-2.
基因/蛋白名称BCL-2
标记Unconjugated
别名PPP1R50, BCL2
数据库入口号Swiss-Prot#: P10415;
NCBI Gene#: 596;
NCBI Protein#: NP_000624.2
Uniprot
P10415
实际分子量26kd
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C/1 year
应用详情
Predicted MW: 26kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela cells using BCL-2 (Phospho-Thr69) Antibody #12036.The lane on the left is treated with the antigen-specific peptide.
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
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