产品详情
产品名称HDAC1 (Phospho-Ser421) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human
特异性The antibody detects endogenous level of HDAC1 only when phosphorylated at Serine 421.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of Serine 421
(E-F-S(p)-D-S) derived from Human HDAC1.
基因/蛋白名称HDAC1
标记Unconjugated
别名HD1, RPD3, GON-10, RPD3L1
数据库入口号Swiss-Prot#: Q13547;
NCBI Gene#: 3065;
NCBI Protein#: NP_004955.2
Uniprot
Q13547
实际分子量62kd
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C/1 year
应用详情
Predicted MW: 62kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HepG2 cells using HDAC1 (Phospho-Ser421) Antibody #12037.The lane on the left is treated with the antigen-specific peptide.
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Component a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
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