产品详情
产品名称Histone H3(Phospho-Ser10) Rabbit Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human;Mouse;Rat
特异性The Histone H3(Phospho Ser10) Rabbit Polyclonal Antibody detects endogenous Histone H3(Phospho Ser10) protein.
免疫原类型peptide
免疫原描述Peptide sequence around phosphorylation site of serine 10(R-K-S(p)-T-G) derived from Human Histone H3.
基因/蛋白名称Histone H3(Phospho-Ser10)
标记Unconjugated
别名H3 histone antibody; HIST1H3A antibody; Histone cluster 1; H3a antibody
数据库入口号Swiss-Prot#:P68431
Uniprot
P68431
实际分子量15-17kd
浓度1.0mg/ml
配方PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1μg/ml, 24 hr; +), using #12084 diluted at 1:1,000.
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
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