产品详情
产品名称TAK1 (Phospho-Thr184) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous levels of TAK1 only when phosphorylated at threonine 184.
免疫原类型peptide
免疫原描述Peptide sequence around phosphorylation site of threonine 184 (I-Q-T(p)-H-M) derived from Human TAK1.
基因/蛋白名称TAK1
标记Unconjugated
别名EC 2.7.11.25; M3K7; MAP3K7; Mitogen-activated protein kinase kinase kinase 7; TGF-beta- activated kinase 1; Transforming growth factor-beta-activated kinase 1; kinase TAK1
数据库入口号Swiss-Prot#:O43318;
NCBI Gene#:6885
Uniprot
O43318
实际分子量65kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using TAK1 (Phospho-Thr184) antibody #12153. The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells (Lane 2), using TAK1 (Phospho-Thr184) Antibody #12153. The lane on the left is treated with synthesized peptide.
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7.
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et al,PGC-1β suppresses saturated fatty acid-induced macrophage inflammation by inhibiting TAK1 activation. In IUBMB Life on 2016 Feb by Hongen Chen, Yan Liu, et al..PMID: 26748475
, (2016),
PMID:
26748475
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