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nrf2(Phospho-S40) Antibody#12811

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产品详情

产品名称nrf2(Phospho-S40) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Affinity purification using immunogen.

应用WB

种属反应性Human;Mouse;Rat

特异性nrf2(Phospho-S40) Antibody detects endogenous levels of nrf2 only when phosphorylated at S40

免疫原类型Peptide

免疫原描述Synthesized peptide derived from human nrf2 around the phosphorylation site of S40.

基因/蛋白名称nrf2(Phospho-S40)

标记Unconjugated

别名NFE2L2, NF-E2-related factor 2, NFE2-related factor 2, NRF2

数据库入口号Swiss-Prot#: Q16236
NCBI Gene ID: 4780

Uniprot Q16236

计算分子量68 kDa

实际分子量80 kDa

浓度1.0mg/mL

配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

Target种属human

应用详情

Western blotting: 1:1000-3000

All lanes: nrf2(Phospho-S40) Rabbit mAb at 1/1k dilution
Lane 1 : HepG2 whole cell lysates
Lane 2 : HepG2 treated with 100ng/ml EGF for 15min whole cell lysates
Lane 3 : Hela whole cell lysates
Lane 4 : Hela treated with 100ng/ml EGF for 15min whole cell lysates
Lane 5 : Mouse brain lysates
Lane 6 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 80 kDa
Exposure time: 7 seconds

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et al,Cocoa ameliorates renal Injury In Zucker diabetic fatty rats by preventIng oxidative stress, apoptosis and Inactivation of autophagy. In Food Funct on 2019 Dec 11 by ?lvarez-Cilleros D , L pez-Oliva ME ,et al..PMID: 31773121 , (2019),
PMID: 31773121
et al,Supplementation with a Cocoa–Carob Blend, Alone or in Combination with Metformin, Attenuates Diabetic Cardiomyopathy, Cardiac Oxidative Stress and Inflammation in Zucker Diabetic Rats. In Antioxidants (Basel) on 2022 Feb 21 by Esther García-Díez, María Elvira López-Oliva, et al..PMID:35204314 , (2022),
PMID: 35204314
et al,Demethylcalabaxanthone from Garcinia mangostana Exerts Antioxidant Effects through the Activation of the Nrf2 Pathway as Assessed via Molecular Docking and Biological EvaluationInAntioxidants (Basel)On2023 Nov 7bySimona De Vita?1,?Milena Masullo et al..PMID:?38001833 , (2023),
PMID: 38001833
et al,Oxidative stress responses and their alterations in the Nrf2-NMDA receptor pathway in the brain of suicide victimsInJ Physiol PharmacolOn2023 JunbyP Panczyszyn-Trzewik et al..PMID:?37661185 , (2023),
PMID: 37661185
et al,Dietary Supplementation with a Cocoa–Carob Blend Modulates Gut Microbiota and Prevents Intestinal Oxidative Stress and Barrier Dysfunction in Zucker Diabetic Rats InAntioxidants (Basel)On2023 AugbyEsther García-Díez,María Elvira López-Oliva et al..PMID:37627514 , (2023),
PMID: 37627514
et al,Cocoa and Carob Supplementation, Alone or in Combination with Metformin, Protects against Hepatorenal Injury in Zucker Diabetic Fatty Rats. In Nutrients on 2024 Sep 13 by Esther García-Díez, María Elvira López-Oliva,et al..PMID:39339687 , (2024),
PMID: 39339687

操作手册

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu人
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk猴
  • Rb兔
  • B牛
  • D狗
  • P猪
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk鸡
;

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