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IRE1 (Phospho-S724) Antibody#13013

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产品详情 图片 产品描述 发表文献(7) 其它
产品详情

产品名称IRE1 (Phospho-S724) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化affinity purified by Protein A

应用WB

种属反应性Human;Mouse;Rat

特异性Phospho-IRE1 (Phospho-S724) Antibody detects endogenous levels of IRE1 only when phosphorylated at phospho S724

免疫原描述KLH conjugated synthesised phosphopeptide derived from human IRE1 around the phosphorylation site of Ser724

基因/蛋白名称ERN1

标记Unconjugated

别名ERN1 | IRE1 (phospho-S724); p-IRE1; phospho-IRE1; p-ERN1; ERN1 | IRE1 (phospho-Ser724); IRE1-BETA; IRE1b; IRE2p; hIRE2p; Ern1; Ire1; mIre1; ERN1_HUMAN; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1 (hIRE1p); Ire1-alpha (IRE1a); ERN1_MOUSE; Inositol-requiring protein 1;

数据库入口号O75460

Uniprot O75460

Gene ID 10595

计算分子量110kDa

浓度1mg/ml

配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20 ˚C.Stable for 12 months from date of receipt

应用详情

WB: 1:500-1:2000

All lanes : IRE1 (Phospho-S724) Antibody at 1/500 dilution
Lane 1 : NIH/3T3 whole cell lysates
Lane 2 : NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysates
Lane 3 : C2C12 whole cell lysates
Lane 4 : C2C12 treated with 100nM Calyculin A for 30 minutes whole cell lysates
Lane 5 : Hela whole cell lysates
Lane 6 : Hela treated with 100nM Calyculin A for 30 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 7 seconds

如果您使用该产品13013发表了文章，请通知我们，让我们可以引用您的文献。

et al,Quercetin Stimulates Mitochondrial Apoptosis Dependent on Activation of Endoplasmic Reticulum Stress in Hepatic Stellate Cells .In Pharm Biol. On 2016 Dec by Liwei He , Xianbang Hou et al..PMID:27572285 , (2016),
PMID: 27572285
et al,Suppressing endoplasmic reticulum stress-related autophagy attenuates retinal light injury. In Aging (Albany NY) on 2020 Aug 28 by Jing-Yao Song, Bin Fan,et al..PMID: 32858529 , (2020),
PMID: 32858529
et al,Endoplasmic reticulum stress is involved in retinal injury induced by repeated transient spikes of intraocular pressure. In J Zhejiang Univ Sci B on 2021 Sept 15 by Xue Yang, Xiaowei Yu et al..PMID: 34514754 , (2021),
PMID: 34514754
et al,Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice. In Int J Mol Sci on 2021 Aug 24 by Danyang Li, Ruqin Lin,et al..PMID:34502057 , (2021),
PMID: 34502057
et al,Didymin Ameliorates Liver Fibrosis by Alleviating Endoplasmic Reticulum Stress and Glycerophospholipid Metabolism: Based on Transcriptomics and Metabolomics. In Drug Des Devel Ther on 2022 Jun 7 by Yan Li, Cuiyu Li, et al..PMID:35698653 , (2022),
PMID: 35698653
et al,Didymin Ameliorates Liver Fibrosis by Alleviating Endoplasmic Reticulum Stress and Glycerophospholipid Metabolism: Based on Transcriptomics and Metabolomics InDrug Des Devel TherOn2022 Jun 7byYan Li , Cuiyu Li et al..PMID:35698653 , (2023),
PMID: 35698653
et al,Artemisinin alleviates astrocyte overactivation and neuroinflammation by modulating the IRE1/NF-κB signaling pathway in in vitro and in vivo Alzheimer's disease models. , (2025),
PMID: 39826816

操作手册

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu人
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk猴
  • Rb兔
  • B牛
  • D狗
  • P猪
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk鸡
;

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