产品详情
产品名称Erk1/Erk2(Phospho-Thr202/185) Rabbit mAb
克隆号SZ2-4
来源种属Recombinant Rabbit
克隆性Monoclonal
亚型IgG
纯化ProA affinity purified
应用WB;ICC/IF;IHC
种属反应性Human
免疫原描述Synthetic phospho-peptide corresponding to residues surrounding Thr185 of human Erk2.
基因/蛋白名称MK03
标记Unconjugated
别名ERK 1 antibody; ERK 2 antibody; ERK-1 antibody; ERK-2 antibody; ERK1 antibody; erk1/2 antibody; ERK2 antibody; ERT1 antibody; ERT2 antibody; Extracellular signal regulated kinase 1 antibody; Extracellular signal-regulated kinase 1 antibody; Extracellular signal-regulated kinase 2 antibody; HS44KDAP antibody; HUMKER1A antibody; Insulin-stimulated MAP2 kinase antibody; MAP kinase 1 antibody; MAP kinase 2 antibody; MAP kinase 3 antibody; MAP kinase isoform p42 antibody; MAP kinase isoform p44 antibody; MAPK 1 antibody; MAPK 2 antibody; MAPK 3 antibody; Mapk1 antibody; MAPK2 antibody; MAPK3 antibody; Microtubule-associated protein 2 kinase antibody; Mitogen-activated protein kinase 1 antibody; Mitogen-activated protein kinase 2 antibody; Mitogen-activated protein kinase 3 antibody; MK01_HUMAN antibody; p38 antibody; p40 antibody; p41 antibody; p41mapk antibody; p42-MAPK antibody; P42MAPK antibody; p44-ERK1 antibody; p44-MAPK antibody; p44ERK1 antibody; p44MAPK antibody; PRKM 2 antibody; PRKM1 antibody; PRKM2 antibody; PRKM3 antibody; protein tyrosine kinase ERK2 antibody
数据库入口号Swiss-Prot#:P27361
Uniprot
P27361
计算分子量41 kDa
实际分子量42,44kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : Erk1/Erk2(Phospho-T202/T185) Rabbit mAb at 1/1k dilutionLane 1 : C6 whole cell lysates Lane 2 : CHO-K1 whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 41 kDa Observed band size: 42,44kDaExposure time: 4 seconds
All lanes : Erk1/Erk2(Phospho-T202/T185) Rabbit mAb at 1/1k dilutionLane 1 : 3T3 whole cell lysatesLane 2 : 3T3 treated with 100ng/mL PDGF for 10 minutes whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 41 kDa Observed band size: 42,44kDaExposure time: 2 seconds
Formalin-fixed;paraffin-embedded human lung cancer tissue stained for Erk1(Phospho-T202)/Erk2(Phospho-T185) using 13340 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded human kidney tissue stained for Erk1(Phospho-T202)/Erk2(Phospho-T185) using 13340 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Erk1(Phospho-T202)/Erk2(Phospho-T185) antibody (13340)
ICC/IF staining of Erk1(Phospho-T202)/Erk2(Phospho-T185) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13340 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit;used at a dilution of 1/500.
The cells were incubated 30 minutes at room temperature with phalloidin tritc .
Nuclei were counterstained with DAPI.
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.
Yes
