Histone H2A.X(Phospho-Ser139) Rabbit mAb#13343
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All lanes : Histone H2A.X(Phospho-Ser139) Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysatesLane 2 : Hela treated with UV for 2 hours whole cell lysatesLane 3 : 293 whole cell lysatesLane 4 : 293 treated with UV for 2 hours whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 15 kDa Observed band size: 15 kDaExposure time: 6 seconds
All lanes : Histone H2A.X(Phospho-Ser139) Rabbit mAb at 1/1k dilutionLane 1 : C6 whole cell lysatesLane 2 : 3T3 whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 15 kDa Observed band size: 15 kDaExposure time: 5 seconds
Formalin-fixed;paraffin-embedded mouse testis tissue stained for Histone H2A.X (Phospho-S139) using 13343 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded rat colon tissue stained for Histone H2A.X (Phospho-S139) using 13343 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Histone H2A.X (Phospho-S139) antibody (13343)
ICC/IF staining of Histone H2A.X (Phospho-S139) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13343 at a working dilution of 1/100. The secondary antibody was Alexa Fluor®647 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
