产品详情
产品名称Src(Phospho-Y419) Rabbit mAb
克隆号ST0800
来源种属Recombinant Rabbit
克隆性Monoclonal
纯化ProA affinity purified
应用WB;ICC/IF
种属反应性Human;Mouse;Rat
免疫原描述Synthetic phospho-peptide corresponding to residues surrounding Tyr419 of human Src.
标记Unconjugated
别名ASV antibody
Avian sarcoma virus antibody
c SRC antibody
CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
cSrc antibody
EC 2.7.10.2 antibody
Neuronal CSRC tyrosine specific protein kinase antibody
Neuronal SRC antibody
Oncogene SRC antibody
OTTHUMP00000174476 antibody
OTTHUMP00000174477 antibody
p60 Src antibody
p60-Src antibody
p60Src antibody
pp60c src antibody
pp60c-src antibody
pp60csrc antibody
Proto oncogene tyrosine protein kinase Src antibody
Proto-oncogene c-Src antibody
Proto-oncogene tyrosine-protein kinase Src antibody
Protooncogene SRC antibody
Protooncogene SRC Rous sarcoma antibody
Src antibody
SRC Oncogene antibody
SRC proto oncogene non receptor tyrosine kinase antibody
SRC_HUMAN antibody
SRC1 antibody
Tyrosine kinase pp60c src antibody
Tyrosine protein kinase SRC 1 antibody
Tyrosine protein kinase SRC1 antibody
v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
数据库入口号Swiss-Prot#:P12931
Uniprot
P12931
计算分子量60 kDa
实际分子量60 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes : Src(Phospho-Y419) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 10 seconds
All lanes : Src (Phospho-Tyr419) Rabbit mAb at 1/1k dilutionLane 1 : MCF7 whole cell lysatesLane 2 : HT-1080 whole cell lysatesLane 3 : HT-1080 treated with Lambda Protein Phosphatase for 30min whole cellLane 4 : Mouse Bladder lysatesLane 5 : Rat Bladder lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 60 kDa Observed band size: 60 kDaExposure time: 5 seconds
Immunocytochemistry/ Immunofluorescence Src(Phospho-Y419) antibody (13367)
ICC/IF staining of Src(Phospho-Y419) in A549 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13367 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells. Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active.
Yes
