Yes
All lanes: SHP2(Phospho-Y542) Rabbit mAb at 1/1k dilution
Lane 1 : NIH/3T3 whole cell lysates
Lane 2 : NIH/3T3 treated with 40ng/mL PDGF for 40 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 15 seconds
Immunocytochemistry/ Immunofluorescence SHP2 (Phospho-Y542) antibody (13393)
ICC/IF staining of SHP2(Phospho-Y542) in B6F1 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13393 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence SHP2 (Phospho-Y542) antibody (13393)
ICC/IF staining of SHP2(Phospho-Y542) in 293 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13393 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
