Yes
All lanes : YAP1 (Phospho-S127) Rabbit mAb at 1/500 dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100nM Calyculin A for 30 minutes whole cell lysates
Lane 3 : C6 whole cell lysates
Lane 4 : C6 treated with 100nM Calyculin A for 30 minutes whole cell lysates
Lane 5 : NIH/3T3 whole cell lysates
Lane 6 : NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 65kDa
Exposure time: 7 seconds
Formalin-fixed;paraffin-embedded human breast carcinoma tissue stained for YAP1 (Phospho-S127) using 13401 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded human kidney tissue stained for YAP1 (Phospho-S127) using 13401 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded mouse kidney tissue stained for YAP1 (Phospho-S127) using 13401 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence YAP1 (Phospho-S127) antibody (13401)
ICC/IF staining of YAP1 (Phospho-S127) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13401 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
