产品详情
产品名称TBK1/NAK (phospho-Ser172) rabbit pAb
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was purified by immunogen affinity chromatography.
应用WB;IHC;IF
种属反应性Human;Mouse;Rat
特异性This antibody detects endogenous levels of Human Mouse TBK1/NAK (phospho-Ser172)
免疫原描述KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S172 of human TBK1 protein.
基因/蛋白名称TBK1
标记Unconjugated
别名NAK; Serine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1
数据库入口号Swiss Prot:Q9UHD2
GeneID:29110
Uniprot
Q9UHD2
Gene ID
29110
计算分子量83kDa
配方Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
保存Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
应用详情
WB 1:500-1000; IHC 1:50-100; IF 1:50-200
Lane 1: Human Raji cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-Phospho-TBK1 (Ser172)at 1/1000 dilution Secondary:Goat anti-Rabbit IgG Secondary Antibody HRP conjugated at 1/20000 dilution Predicted band size: 84 kDa Observed band size: 84 kDa
Western blot analysis of TBK1 (Phospho-S172) expression in rat testis (A), SGC7901 (B), EC9706 (C), A549 (D), 3T3L1 (E) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 83 kD)
Immunohistochemical analysis of TBK1 (Phospho-S172) staining in human ovarian cancer formalin fixed paraffin embedded tissue section.
Immunofluorescent analysis of TBK1 (Phospho-S172) staining in MDAMB231 cells.
TANK binding kinase 1(TBK1) Homo sapiens The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010],
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et al,Activation of cGAS-STING pathway – A possible cause of myofiber atrophy/necrosis in dermatomyositis and immune-mediated necrotizing myopathy. In J Clin Lab Anal on 2022 Oct by Meichen Zhou, Xiaoxiao Cheng, et al..PMID:36030554
, (2022),
PMID:
36030554
Yes
