产品详情
产品名称5-LO (Phospho-Ser523) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB;ELISA
种属反应性Human;Rat
特异性Phospho-5-LO (S523) Polyclonal Antibody detects endogenous levels of 5-LO protein only when phosphorylated at S523.
免疫原描述Synthesized phospho-peptide around the phosphorylation site of human 5-LO (phospho Ser523)
标记Unconjugated
别名ALOX5; LOG5; Arachidonate 5-lipoxygenase; 5-LO; 5-lipoxygenase
数据库入口号Swiss Prot:P09917
GeneID:240
Uniprot
P09917
计算分子量78 kDa
实际分子量78 kDa
浓度1 mg/ml
配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
保存-20˚C/1
应用详情
Western Blot: 1/500 - 1/2000.
ELISA: 1/10000. Not yet tested in other applications.
All lanes : 5-LO (Phospho-Ser272) Antibody at 1/500 dilution Lane 1 : Rat heart lysate Lane 2 : Rat kidney lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 78 kDa Observed band size: 78 kDa Exposure time: 8 seconds
All lanes : 5-LO (Phospho-Ser523) Antibody at 1/500 dilution Lane 1 : JK whole cell lysates Lane 2 : JK treated with Lambda Protein Phosphatase for 1 hour whole cell Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 78 kDa Observed band size: 78 kDa Exposure time: 6 seconds
arachidonate 5-lipoxygenase(ALOX5) Homo sapiens This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],
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Yes
