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位置: 首页 > KO/KD抗体 > Smad2 (Phospho-Ser250) Rabbit mAb

Smad2 (Phospho-Ser250) Rabbit mAb#14211

是否有货: Yes
产品详情

产品名称Smad2 (Phospho-Ser250) Rabbit mAb

来源种属Recombinant Rabbit

克隆性Monoclonal

亚型Rabbit IgG

纯化Affinity-chromatography

应用WB

种属反应性Human;Mouse;Rat

特异性Phospho-Smad2 (S250) Antibody detects endogenous levels of Phospho-Smad2 (S250)

免疫原描述A synthesized peptide derived from human Smad2

标记Unconjugated

别名JV18-1, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, Smad 2;

数据库入口号Uniprot:Q15796

Uniprot Q15796

计算分子量52 kDa

实际分子量58 kDa

配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

应用详情

WB:1:1000~1:2000
FC:1:20

All lanes: Smad2 (Phospho-Ser250) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with alkaline phosphatase whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa
Exposure time: 7 seconds
All lanes: Smad2 (Phospho-Ser250) Rabbit mAb at 1/1k dilution
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa
Exposure time: 10 seconds
All lanes :Smad2 (Phospho-Ser250) Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 : Smad2 (Phospho-Ser250) knockdown HT-1080 cell lysate
Lysates/proteins at 20 µg per lane.

如果您使用该产品14211发表了文章,请通知我们,让我们可以引用您的文献。

et al,Characterization of miR-34a-Induced Epithelial–Mesenchymal Transition in Non-Small Lung Cancer Cells Focusing on p53 , (2021),
PMID: 34944497

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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