Yes
All lanes: CDC37 (Phospho-Ser13) Rabbit mAb at 1/1k dilution
Lane 1 : JK treated with alkaline phosphatase whole cell lysates
Lane 2 : JK whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 50 kDa
Exposure time: 4 seconds
All lanes: CDC37 (Phospho-Ser13) Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : PC12 whole cell lysatesLane 3 : 293 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 50 kDa
Exposure time: 7 seconds
All lanes:CDC37 (Phospho-Ser13) Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : CDC37 (Phospho-Ser13) knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human brain tissue stained for CDC37 (Phospho-Ser13) using 14226 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat brain tissue stained for CDC37 (Phospho-Ser13) using 14226 at 1/100 dilution in immunohistochemical analysis.
