STAT3 (Phospho-Tyr705) Rabbit mAb#14235
评价
Yes
All lanes: STAT3 (Phospho-Tyr705) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100ng/ml IFNa for 5min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 7 seconds
All lanes: STAT3 (Phospho-Tyr705) Rabbit mAb at 1/1k dilution
Lane 1 : Mouse lung lysates
Lane 2 : Mouse kidney lysatesLane 3 : Rat lung lysatesLane 2 : Rat skin lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 11 seconds
All lanes:STAT3 (Phospho-Tyr705) Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : STAT3 (Phospho-Tyr705) knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human pancreas tissue stained for STAT3 (Phospho-Tyr705) using 14235 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence STAT3 (Phospho-Tyr705) antibody (14235) ICC/IF staining of STAT3 (Phospho-Tyr705) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 14235 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
