Yes
All lanes : Phospho-GSK3 beta (S9) Rabbit mAb at 1/1k dilution Lane 1 : Hela whole cell lysates Lane 2 : 293T whole cell lysates Lane 3 : MCF7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 46 kDa Exposure time: 7 seconds
All lanes : Phospho-GSK3 beta (S9) Rabbit mAb at 1/1k dilution Lane 1 : 293T whole cell lysates Lane 2 : 293T treated with Lambda Protein Phosphatase for 30min whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 46 kDa Exposure time: 3 seconds
All lanes : Phospho-GSK3 beta (S9) Rabbit mAb at 1/1k dilution Lane 1 : Wild-type Hela cell lysate Lane 2 : Phospho-GSK3 beta (S9) knockdown Hela cell lysate Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Phospho-GSK3 beta (S9) using 14237 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence p-GSK3 beta (S9) antibody (14237) 14237 staining p-GSK3 beta (S9) in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 14237 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 14237 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.
