eIF4B (Phospho-Ser406) Rabbit mAb#14278
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All lanes : Phospho-eIF4B (S406) Rabbit mAb at 1/1k dilution Lane 1 : Hela whole cell lysates Lane 2 : 293T whole cell lysates Lane 3 : K562 whole cell lysates Lane 4 : HepG2 whole cell lysates Lane 5 : 3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time: 3 seconds
All lanes : Phospho-eIF4B (S406) Rabbit mAb at 1/1k dilution Lane 1 : Mouse Liver lysates Lane 2 : Rat Liver lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time: 3 seconds
All lanes : Phospho-eIF4B (S406) Rabbit mAb at 1/1k dilution Lane 1 : Hela whole cell lysates Lane 2 : Hela treated with 100nM Insulin for 20min whole cell lysates Lane 3 : 293T whole cell lysates Lane 4 : 293T treated with Lambda Protein Phosphatase for 30min whole cell lysates Lane 5 : 293T treated with 100nM Insulin for 20min whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time: 6 seconds
Formalin-fixed, paraffin-embedded mouse lung tissue stained for Phospho-eIF4B (S406) using 14278 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat lung tissue stained for Phospho-eIF4B (S406) using 14278 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence p-eIF4B (S406) antibody (14278) 14278 staining p-eIF4B (S406) in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 14278 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 14278 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.
