NF-κB p65 (Phospho-Ser536) Rabbit mAb#14319
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All lanes : NF-κB p65 (Phospho-Ser536) Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysatesLane 2 : Hela treated with 20ng/ml TNF-α for 15 minutes whole cell lysatesLane 3 : C2C12 whole cell lysatesLane 4 : C6 whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 60 kDa Observed band size: 65 kDaExposure time: 10 seconds
Immunocytochemistry/Immunofluorescence NF-κB p65 (Phospho Ser536) antibody (14319)
ICC/IF staining of NF-κB p65 (Phospho Ser536) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14319 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
