产品详情
产品名称PDK1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total PDK1 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.239~243 (A-N-S-F-V) derived from Human PDK1.
基因/蛋白名称PDK1
标记Unconjugated
别名PDPK1, PkB kinase, Protein kinase B kinase, hPDK1, kinase PDK1
数据库入口号Swiss-Prot: O15530
NCBI Protein: NP_002604.1
Uniprot
O15530
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 63kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from MCF, 293 and PC12 cells using PDK1(Ab-241) Antibody #21005.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1(Ab-241) Antibody #21005(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using PDK1(Ab-241) Antibody #21005.
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development
Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63
Chen H, et al. (2001) Biochemistry; 40(39): 11851-9
Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7
Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11
Beausoleil SA, et al. (2004)Proc Natl Acad Sci U S A; 101(33): 12130-5
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et al,PDK1-Foxo1 in Agouti-Related Peptide Neurons Regulates Energy Homeostasis by Modulating Food Intake and Energy Expenditure
, (2011),
PMID:
21694754
et al,AXL phosphorylates and up-regulates TNS2 and its implications in IRS-1-associated metabolism in cancer cells.In J Biomed Sci On 2018 Nov 12 By Li-Chun Cheng, Yen-Lin Chen et al..PMID: 30419905
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et al,Activation of mammalian target of rapamycin pathway confers adverse outcome in nonsmall cell lung carcinoma锟?In Cancer on 2011
Aug 15 by Dan Liu, Yi Huang, et al..PMID:21387259
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et al,Phosphoproteome analysis reveals an important role for glycogen synthase kinase-3 in perfluorododecanoic acid-induced rat liver toxicity. In Toxicol Lett on 2013 Mar 27 by Hongxia Zhang, Junjie Hou, et al..PMID: 23353032
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PMID:
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et al,PDK1-Foxo1 in agouti-related peptide neurons regulates energy homeostasis by modulating food intake and energy expenditure. In PLoS One on 2011 by Yongheng Cao, Masanori Nakata, et al..PMID: 21694754
, (2011),
PMID:
21694754
et al,Notch-induced hIL-6 production facilitates the maintenance of self-renewal of hCD34+ cord blood cells through the activation of Jak-PI3K-STAT3 pathway. In Am J Pathol
on 2012 Jan by Bongkum Choi, Eunyoung Chun, et al..PMID:22062221
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