产品详情
产品名称Raf1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total Raf1 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.257~261 (S-T-S-T-P) derived from Human RAF.
基因/蛋白名称Raf1
标记Unconjugated
别名C-RAF; C-Raf; CRAF
数据库入口号Swiss-Prot: P04049
NCBI Protein: NP _002871.1
Uniprot
P04049
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 73kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela, K562 and HT29 cells using Raf1(Ab-259) Antibody #21006.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Ab-259) Antibody #21006.
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.
Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468.
Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283.
Chong H, et al. (2001) EMBO J. 20, 3716-3727.
King A J, et al. (1998) Nature. 396:180-183.
Fabian J R, et al. (1993) Mol Cell Biol. 13: 7170-7179.
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et al,Prognostic Impact of Raf-1 and p-Raf-1 Expressions for Poor Survival Rate in Non-Small Cell Lung Cancer.In Cancer Sci on 2012 Oct by Zhi-Xin Qiu, Lei Wang,et al..PMID:22738312
, (2012),
PMID:
22738312
et al,MicroRNA-146b, a sensitive indicator of mesenchymal stem cell repair of acute renal injury. In Stem Cells Transl Med on 2016 Oct by Yuan Zhu, Jing Yu, et al..PMID: 27400799
, (2016),
PMID:
27400799
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