产品详情
产品名称NFkB-p65 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total NFkB-p65 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.534~538 (F-S-S-I-A) derived from Human NFkB-p65.
基因/蛋白名称NFkB-p65
标记Unconjugated
别名NFKB3; RELA; TF65; Transcription factor p65; p65
数据库入口号Swiss-Prot: Q04206
NCBI Protein: NP_001138610.1
Uniprot
Q04206
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:50-1:500;
Western blot analysis of extracts from Hela and 293 cells using NFkB-p65 Antibody.
Western blot analysis of extracts from HT29, HL60 and COLO cells using NFkB-p65 Antibody.
Western blot analysis of extracts from NIH/3T3 and PC-12 cells using NF-kB p65 antibody.
Immunohistochemical analysis of paraffin-embedded human breast cancer using NFkB-p65 Antibody.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using NFkB-p65 Antibody.
Immunohistochemistry of paraffin-embedded mouse lung using NF-kB p65 Rabbit pAb.
Immunofluorescence staining of PC-3 cells with NFkB-p65 Antibody at 1:200, counter-stained with DAPI.
Immunofluorescence analysis of PC-12 cells using NF-kB p65 Rabbit pAb at a dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using NF-kB p65 Rabbit pAb.
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex
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Haskill S, et al. (1991) Cell 65:1281-1289.
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