产品详情
产品名称c-Jun Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total c-Jun protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.89~93 (T-T-T-P-T) derived from Human c-Jun.
基因/蛋白名称c-Jun
标记Unconjugated
别名AH119; AP1; Jun A; c-Jun; p39
数据库入口号Swiss-Prot: P05412
NCBI Protein: NP_002219.1
Uniprot
P05412
计算分子量36kDa
实际分子量39-48kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000;
IHC 1:50-1:200;
IF 1:50-1:200;
Western blot analysis of extracts from HT19, HepG2, 293 and Hela cells using c-Jun Antibody.
Immunohistochemistry of paraffin-embedded Human cervical carcinoma tissue using c-Jun Antibody .
Immunohistochemistry of paraffin-embedded Mouse brain tissue using c-Jun Antibody .
Immunohistochemistry of paraffin-embedded Rat brain tissue using c-Jun Antibody .
Immunohistochemistry of paraffin-embedded Rat skin tissue using c-Jun Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence analysis of Rat skin tissue using c-Jun Antibody .
Immunofluorescence analysis of HEK293 cells using c-Jun Antibody .Picture A: c-Jun antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B
Immunofluorescence analysis of A549 cells using c-Jun Antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using c-Jun Antibody. Blue: DAPI for nuclear staining.
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
Akira Ikari, Chiaki Okude, Hayato Sawada, et al. (2008) Down-regulation of TRPM6-mediated magnesium influx by cyclosporin A Naunyn-Schmiedeberg's Archives of Pharmacology, 377(4-6):333-43.
This article references the use of the #21021 in the following applications :Western blotting
如果您使用该产品21021发表了文章，请通知我们，让我们可以引用您的文献。
et al,Down-regulation of TRPM6-mediated magnesium influx by cyclosporin A Naunyn-Schmiedeberg's.
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