产品详情
产品名称CREB Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC
种属反应性Human;Mouse
特异性The antibody detects endogenous level of total CREB protein and it also detects the CREB-related protein ATF-1.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 131~135 (R-P-S-Y-R) derived from Human CREB.
基因/蛋白名称CREB
标记Unconjugated
别名CREB-1; CREB1;
数据库入口号Swiss-Prot: P16220
NCBI Protein: NP _004370.1
Uniprot
P16220
计算分子量35kDa
实际分子量43kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293, HT29, Hela and 3T3 cells using CREB Antibody.
Western Blot analysis of extracts from KB cells using CREB Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB Antibody (left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using CREB Antibody at dilution of 1:50 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Mouse brain using CREB Antibody at dilution of 1:50 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CREB1 antibody.
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55.
Tan Y, et al.( 1996) EMBO J; 15(17): 4629-42.
Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.
Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.
Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.
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