产品详情
产品名称PTEN Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total PTEN protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.378~382/380~384/381~385 (R-Y-S-D-T-T-D-S) derived from Human PTEN.
基因/蛋白名称PTEN
标记Unconjugated
别名MMAC1; Mutated in multiple advanced cancers 1; Protein-tyrosine phosphatase PTEN; TEP1;
数据库入口号Swiss-Prot: P60484
NCBI Protein: NP_000305.3
Uniprot
P60484
计算分子量47kDa
实际分子量54kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000; IHC 1:50-1:200;
Western blot analysis of extracts from various cell lines using PTEN Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PTEN Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemical analysis of paraffin-embedded human colon cancer using PTEN Antibody.
Immunohistochemical analysis of paraffin-embedded human lung cancer using PTEN Antibody.
Immunohistochemical analysis of paraffin-embedded Human panreas using PTEN Antibody.
Immunohistochemical analysis of paraffin-embedded Rat panreas using PTEN Antibody.
Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.
Al-Khouri AM, et al. (2005) J Biol Chem. 280(42):35195-35202.
Torres J, et al. (2001) J Biol Chem. 276(2): 993-998.
Vazquez F, et al. (2000) Mol Cell Biol. 20(14): 5010-5018.
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