产品详情
产品名称BAD Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC
种属反应性Human
特异性The antibody detects endogenous level of total BAD protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.153~157 (R-M-S-D-E) derived from Mouse BAD.
基因/蛋白名称BAD
标记Unconjugated
别名Bbc2
数据库入口号Swiss-Prot: Q61337
NCBI Protein: NP_031548.1
Uniprot
Q61337
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 23kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells using BAD(Ab-155) Antibody #21064 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Ab-155) Antibody #21064(left) or the same antibody preincubated with blocking peptide(right).
The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Moon EY, et al. (2003). Blood.101 (10): 4122-4130.
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et al,Loss of Bad expression confers poor prognosis in non-small cell lung cancer
, (2012),
PMID:
21918885
et al,Loss of Bad expression confers poor prognosis in non-small cell lung cancer. In Med Oncol on 2012 Sep by Huang Y, Liu D,
et al..PMID:21918885
, (2012),
PMID:
21918885
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