产品详情
产品名称Estrogen Receptor-a Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC
种属反应性Human
特异性The antibody detects endogenous level of total Estrogen Receptor-a protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.102~106 (S-V-S-P-S) derived from Human Estrogen Receptor-a.
基因/蛋白名称Estrogen Receptor-a
标记Unconjugated
别名ER; ESR; ESR1; ESTR; ESTRA
数据库入口号Swiss-Prot: P03372
NCBI Protein: NP_000116.2
Uniprot
P03372
计算分子量66kDa
实际分子量66kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 66kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of lysates from HepG2 and COLO cells using Estrogen Receptor-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of extracts from Hela, 293 and MCF7 cells using Estrogen Receptor-a Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-a Antibody.
Immunohistochemistry analysis of paraffin-embedded Human Uterus tissue using Estrogen Receptor-a Antibody.
Immunohistochemistry analysis of paraffin-embedded Human esophagus cancer tissue using Estrogen Receptor-a Antibody.
Immunohistochemistry analysis of paraffin-embedded Human ovarian cancer tissue using Estrogen Receptor-a Antibody.
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014.
Dutertre M, et al. (2003). Mol Endocrinol.17 (7): 1296-1314.
Chen D, et al. (2000). Mol Cell.6 (1): 127-137.
Rogatsky I, et al. (1999). J Biol Chem.274 (32): 22296-22302.
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et al,Serum-free mouse embryo cells generate a self-sustaining feedback loop for an astrocyte marker protein and respond to cytokines and bisphenol A in accordance with the subtle difference in their differentiation state.
, (2007),
PMID:
17210262
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