产品详情
产品名称Estrogen Receptor-a Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total Estrogen Receptor-a protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.104~108 (S-P- S-P-L) derived from Human Estrogen Receptor-a.
基因/蛋白名称Estrogen Receptor-a
标记Unconjugated
别名ER; ESR; ESR1; ESTR; ESTRA
数据库入口号Swiss-Prot: P03372
NCBI Protein: NP_000116.2
Uniprot
P03372
计算分子量66kDa
实际分子量66kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000; IHC 1:50-1:200; IF 1:50-1:200;
Western blot analysis of extracts from MCF7 cells using Estrogen Receptor-a Antibody and the same antibody preincubated with blocking peptide.
Western blot analysis of extracts from Mouse brain tissue, 231 and Hela cells using Estrogen Receptor-a Antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded mammary cancer tissues with DAB staining using Estrogen Receptor-a Antibody.
Immunohistochemistry analysis of paraffin-embedded rat uterus tissues using Estrogen Receptor-a Antibody.
Immunohistochemistry analysis of paraffin-embedded Rat ovary tissues using Estrogen Receptor-a Antibody.
Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a Antibody.
Immunofluorescence analysis of Hela cells using Estrogen Receptor-a Antibody (green).
Immunofluorescence analysis of C6 cells using Estrogen Receptor-a Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Estrogen Receptor-a Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2OS cells using Estrogen Receptor-a Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014.
Dutertre M, et al. (2003). Mol Endocrinol.17 (7): 1296-1314.
Chen D, et al. (2000). Mol Cell.6 (1): 127-137.
Rogatsky I, et al. (1999). J Biol Chem.274 (32): 22296-22302.
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et al,Analysis of prognostic factors and treatment of anaplastic meningioma in China. In J Clin Neurosci on 2015 Apr by Qing Xie, Yu Zhou, et al..PMID:25744075
, (2015),
PMID:
25744075