产品详情
产品名称ASK1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC IF
种属反应性Human
特异性The antibody detects endogenous level of total ASK1 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.81~85 (G-S-S-V-G) derived from Human ASK1.
基因/蛋白名称ASK1
标记Unconjugated
别名ASK-1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5
数据库入口号Swiss-Prot: Q99683
NCBI Protein: NP_005914.1
Uniprot
Q99683
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from MDA-MB- 435 cells using ASK1(Ab-83) Antibody #21125 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Ab-83) Antibody #21125(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using ASK1(Ab-83) Antibody #21125.
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
Mabuchi S, et al. (2004) Endocrinology. 145(1): 49-58.
Yuan ZQ, et al. (2003) J Biol Chem. 278(26): 23432-23440.
Kim AH, et al. (2001) Mol Cell Biol. 21(3): 893-901.
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et al,ASK1鈥揓NK signaling cascade mediates Ad鈥怱T13鈥恑nduced apoptosis in colorectal HCT116 cells. In J Cell Biochem on 2010 Jun 1 by Min Yang, Mingcan Yu, et al..PMID:20512919
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PMID:
20512919
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