产品详情
产品名称BRCA1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IF
种属反应性Human
特异性The antibody detects endogenous level of total BRCA1 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.1522~1526 (Y-P-S-Q-E) derived from Human BRCA1.
基因/蛋白名称BRCA1
标记Unconjugated
别名RNF53
数据库入口号Swiss-Prot: P38398
NCBI Protein: NP_009225.1
Uniprot
P38398
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 220kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 and MCF cells using BRCA1(Ab-1524) Antibody #21139.
Immunofluorescence staining of methanol-fixed Hela cells using BRCA1(Ab-1524) Antibody #21139.
The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation
Lin SY, et al. (2004) Proc Natl Acad Sci U S A, 101(17): 6484-6489
Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718
Beger C, et al. (2001) Proc Natl Acad Sci U S A; 98(1): 130-135
Gardner K, et al. (2001) Breast Cancer Res; 3(1): 11-13
Zheng L, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9587-9592
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et al,BRCA1 is required for meiotic spindle assembly and spindle assembly checkpoint activation in mouse oocytes. In Biol Reprod on 2008 Oct by Bo Xiong, Sen Li, et al..PMID:18596218
, (2008),
PMID:
18596218
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