产品详情
产品名称HSP27 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC
种属反应性Human
特异性The antibody detects endogenous level of total HSP27 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.13~17 (G-P-S-W-D) derived from Human HSP27.
基因/蛋白名称HSP27
标记Unconjugated
别名28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; HSP 27; HSP25
数据库入口号Swiss-Prot: P04792
NCBI Protein: NP _001531.1
Uniprot
P04792
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells using HSP27(Ab-15) Antibody #21144 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Ab-15) Antibody #21144(left) or the same antibody preincubated with blocking peptide(right).
Involved in stress resistance and actin organization.
如果您使用该产品21144发表了文章，请通知我们，让我们可以引用您的文献。
et al,Upregulation of heat shock protein 27 confers resistance to actinomycin D鈥恑nduced apoptosis in cancer cells. In FEBS J on 2013 Sep by Wenbo Ma, Yan Teng,et al..PMID: 23848600
, (2013),
PMID:
23848600
et al,Anaplastic astrocytomas with abundant R osenthal fibers in elderly patients: A diagnostic pitfall of high鈥恎rade gliomas. In Neuropathology on 2013 Oct by Yasuo Sugita, Shinji Nakashima, et al..PMID: 23438297
, (2013),
PMID:
23438297
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