产品详情
产品名称cdc25C Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC IF
种属反应性Human
特异性The antibody detects endogenous level of total cdc25C protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.214~218 (S-P-S-M-P) derived from Human cdc25C.
基因/蛋白名称cdc25C
标记Unconjugated
别名CDC25M1; MPIP3;
数据库入口号Swiss-Prot: P30307
NCBI Protein: NP_001781.2
Uniprot
P30307
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from HT29 cells using cdc25C(Ab-216) Antibody #21145 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C(Ab-216) Antibody #21145(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using cdc25C(Ab-216) Antibody #21145.
cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded
protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3(4): 341-348.
Ferguson AM. et al. (2005) Mol Cell Biol. 25(7): 2853-2860.
Donzelli M. et al. (2003) EMBO Rep. 4(7): 671-677.
Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99(4): 1990-1995.
如果您使用该产品21145发表了文章，请通知我们，让我们可以引用您的文献。
et al,MEF2 is regulated by CaMKII未2 and a HDAC4鈥揌DAC5 heterodimer in vascular smooth muscle cells. In Biochem J on 2012 May 15 by Roman Ginnan, Li Yan Sun, et al..PMID:22360269
, (2012),
PMID:
22360269
et al,PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis.In Nat Commun on 2016 Aug 3 by Ji Liang , Ruixiu Cao et al..PMID: 27485204
, (2016),
PMID:
27485204
et al,LncCCAT1 interaction protein PKM2 upregulates SREBP2 phosphorylation to promote osteosarcoma tumorigenesis by enhancing the
Warburg effect and lipogenesis. In Int J Oncol on 2022 Apr by Feifei Pu, Jianxiang Liu,et al..PMID:35244192
, (2022),
PMID:
35244192
有货
