产品详情
产品名称FAK Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total FAK protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.923~927 (K-V-Y-E-N) derived from Human FAK.
基因/蛋白名称FAK
标记Unconjugated
别名FADK 1; FAK1; PTK2
数据库入口号Swiss-Prot: Q05397
NCBI Protein: NP_005598.3
Uniprot
Q05397
计算分子量119kDa
实际分子量125kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000; IHC 1:50-1:200; IF 1:50-1:200;
Western blot analysis of extracts from Rat brain, MCF7 and Hela cells using FAK Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FAK Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FAK Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemistry analysis of paraffin-embedded Rat kidney using FAK Antibody at dilution of 1:50 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Rat spleen using FAK Antibody.
Immunofluorescence staining of methanol-fixed Hela cells using FAK Antibody.
Immunofluorescence staining of methanol-fixed HEK293 cells using FAK Antibody.
Immunofluorescence analysis of RAW264.7 cells using FAK antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Sanders MA, et al. (2005) J Biol Chem; 280(25): 23516-22.
Cherubini A, et al. (2005) Mol Biol Cell; 16(6): 2972-83.
Toriumi Y, et al. (2003) FEBS Lett; 553(3): 419-22.
Shi Q, et al. (2003) Mol Biol Cell; 14(10): 4306-15.
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