产品详情
产品名称Akt2 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total Akt2 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 472~476 (Q-F-S-Y-S) derived from Human Akt2.
基因/蛋白名称Akt2
标记Unconjugated
别名PKB beta; Akt-2; Protein kinase B; RAC-PK-beta;
数据库入口号Swiss-Prot: P31751
NCBI Protein: NP _001617.1
Uniprot
P31751
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 and PC12 cells using Akt2(Ab-474) Antibody #21155.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2(Ab-474) Antibody #21155(left) or the same antibody preincubated with blocking peptide(right).
General protein kinase capable of phosphorylating several known proteins.
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et al,Girdin/GIV is upregulated by cyclic tension, propagates mechanical signal transduction, and is required for the cellular proliferation and migration of MG-63 cells.In Biochem Biophys Res Commun.On 2015 Aug 21 by Hu JT, Li Y et al..PMID:26163263
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