产品详情
产品名称PAK1 Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF;ELISA
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total PAK1protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 210~214 (P-V-T-P-T) derived from Human PAK1.
基因/蛋白名称PAK1
标记Unconjugated
别名p21-activated kinase 1
数据库入口号Swiss-Prot: Q13153
NCBI Protein: NP_001122092.1
Uniprot
Q13153
计算分子量61kDa
实际分子量61-68kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:100-1:300;ELISA 1:5000-1:20000;
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using PAK1 Polyclonal Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemistry analysis of paraffin-embedded Human brain tissue using PAK1 Polyclonal Antibody.
Immunohistochemistry analysis of paraffin-embedded Human hepatocellular carcinoma tissue using PAK1 Polyclonal Antibody.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using PAK1 Polyclonal Antibody.
Immunofluorescence staining of methanol-fixed Hela cells using PAK1 Polyclonal Antibody.DAPI for nuclear staining.
Immunofluorescence staining of methanol-fixed HEK293 cells using PAK1 Polyclonal Antibody.Picture A:Target (red). Picture B: DAPI (blue). Picture C: Merge of A+B
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Alexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819
Thiel DA, et al. (2002) Curr Biol; 12:1227-1232
Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 - 49052.
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et al,Phosphorylation of histone H3 on Ser10 by auto-phosphorylated PAK1 is not essential for chromatin condensation and meiotic progression in porcine oocytes
, (2013),
PMID:
23521812
et al, PAK1 Regulates Spindle Microtubule Organization During Oocyte Meiotic Maturation.In Front Biosci (Elite Ed) on 2010 Jun 1 by Sheng-Li Lin , Shu-Tao Qi,et al..PMID: 20515799
, (2010),
PMID:
20515799
et al,Uptake of Shiga鈥恡oxigenic Escherichia coli鈥匰ubAB by HeLa cells requires an actin锟?and lipid raft鈥恉ependent pathway.In Cell Microbiol on 2014 Oct by Sayaka Nagasawa 1 , Kohei Ogura et al..PMID:24844382
, (2014),
PMID:
24844382
et al,PAK1 Is Involved in the Spindle Assembly during the First Meiotic Division in Porcine OocytesInInt J Mol SciOn2023 Jan 6byLei Peng?1,?Yijing He et al..PMID: 36674642
, (2023),
PMID:
36674642
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