产品详情
产品名称MEK1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total MEK1 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.219~223 (A-N-S-F-V) derived from Human MEK1.
基因/蛋白名称MEK1
标记Unconjugated
别名ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1
数据库入口号Swiss-Prot: Q02750
NCBI Protein: NP_002746.1
Uniprot
Q02750
计算分子量43, 44kDa
实际分子量44kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000; IHC 1:50-1:200;
Western blot analysis of extracts from 293 cells using MEK1 Antibody.
Western blot analysis of extracts from Mouse lung, Mouse stomach and Mouse brain using MEK1 Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue using MEK1 Antibody.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using MEK1 Antibody.
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Zebisch A, et al. (2006) Cancer Res; 66(7): 3401-8.
Luciano BS, et al. (2006)J Biol Chem; 279(50): 52117-23.
Wang X, et al. (2003) Oncogene; 22(1): 109-16.
Gopalbhai K, et al. (2003) J Biol Chem; 278(10): 8118-25.
Ling MT, et al. (2002)Oncogene; 21(55): 8498-505.
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et al,MicroRNA-146b, a sensitive indicator of mesenchymal stem cell repair of acute renal injury. In Stem Cells Transl Med on 2016 Oct by Yuan Zhu, Jing Yu, et al..PMID: 27400799
, (2016),
PMID:
27400799
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