产品详情
产品名称FLT3 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IF
种属反应性Human
特异性The antibody detects endogenous level of total FLT3 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3.
基因/蛋白名称FLT3
标记Unconjugated
别名CD135; FLK-2; FLT-3; FLT3; STK-1
数据库入口号Swiss-Prot: P36888
NCBI Protein: NP_004110.2
Uniprot
P36888
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 130 160 kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 and MCF cells using FLT3(Ab-591) Antibody #21187.
Immunofluorescence staining of methanol-fixed Hela cells using FLT3(Ab-591) Antibody #21187.
FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Sekine S et,al. (2008) J Immunol. ;180(12):8126-34
Pratz K et,al. (2008) Leuk Lymphoma. 2008 ;49(5):852-63.
Al Shaer L et,al. (2008) Br J Haematol. 141(4):483-93.
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et al,Versatile Immunosensor Using a Quantum Dot Coated Silica Nanosphere as a Label for Signal Amplification.
, (2010),
PMID:
20597496
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