产品详情
产品名称MEK1/MEK2 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total MEK1/MEK2 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.215~219/219~223 (I-D-S-M-A) derived from Human MEK1/MEK2.
基因/蛋白名称MEK1/MEK2
标记Unconjugated
别名ERK activator kinase 1/ERK activator kinase2; MAP kinase kinase 1/MAP kinase kinase 2; MAP2K1/MAP2K1; MAPK/ERK kinase 1/MAPK/ERK kinase 2; MAPKK 1/MAPKK 1
数据库入口号Swiss-Prot: Q02750
NCBI Protein: NP_002746.1 NP_109587.1
Uniprot
Q02750
计算分子量43, 44kDa
实际分子量44kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000; IHC 1:50-1:200; IF 1:50-1:200;
Western blot analysis of extracts from U-87MG cells, Mouse brain and Rat brain using MEK1/MEK2 Antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MEK1/MEK2 Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using MEK1/MEK2 Antibody.Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat brain tissue using MEK1/MEK2 Antibody.Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma using MEK1/MEK2 Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence staining of methanol-fixed Hela cells using MEK1/MEK2 Antibody.
Immunofluorescence staining of methanol-fixed U2OS cells using MEK1/MEK2 Antibody.
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Shen R, et al. (2002) Mol Cell Biol; 22(10): 3230-3236
Preisinger C, et al. (2005) EMBO J; 24(4): 753-765
Laine P, et al. (2000) Biochem J; 349(Pt 1): 19-25
Yaglom J, et al. (2003) Mol Cell Biol; 23(11): 3813-3824
Dahan S, et al. (2002) Infect Immun; 70(5): 2304-2310
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