产品详情
产品名称HSF1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC
种属反应性Human
特异性The antibody detects endogenous level of total HSF1 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 305~309 (P-Q-S-P-R) derived from Human HSF1.
基因/蛋白名称HSF1
标记Unconjugated
别名HSTF 1
数据库入口号Swiss-Prot: Q00613
NCBI Protein: NP_005517.1
Uniprot
Q00613
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 82kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela, HepG2 and A2780 cells using HSF1(Ab-307) Antibody #21206.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1(Ab-307) Antibody #21206(left) or the same antibody preincubated with blocking peptide(right).
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4
Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026
Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.
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et al,Solanesol protects human hepatic L02 cells from ethanol-induced oxidative injury via upregulation of HO-1 and Hsp70.In Toxicol In Vitro on 2015 Apr by Xiangyang Yao, Qin Bai et al..PMID:25645596
, (2015),
PMID:
25645596
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