产品详情
产品名称b-Catenin Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total b-Catenin protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 35~39 (I-H-S-G-A) derived from Human b-Catenin.
基因/蛋白名称b-Catenin
标记Unconjugated
别名CTNNB1; CATNB; CTNB1; CTNNB;
数据库入口号Swiss-Prot: P35222
NCBI Protein: NP_001091679.1
Uniprot
P35222
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 92kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from HT29 and SKOV3 cells using b-Catenin(Ab-37) Antibody #21212.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Ab-37) Antibody #21212(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using b-Catenin(Ab-37) Antibody #21212.
Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
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