产品详情
产品名称IRS-1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total IRS-1 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.634~638 (P-M-S-P-K) derived from Human IRS-1.
基因/蛋白名称IRS-1
标记Unconjugated
别名IRS-1; IRS1;
数据库入口号Swiss-Prot: P35568
NCBI Protein: NP_005535.1
Uniprot
P35568
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 180kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells using IRS-1(Ab-636) Antibody #21223.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1(Ab-636) Antibody #21223(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using IRS-1(Ab-636) Antibody #21223.
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76
Kadowaki T, et al. (2000) J Clin Invest; 106(4): 459-465
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97(5): 2355-2360
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et al,The role of mitochondrial oxidative stress in the metabolic alterations in diet-induced obesity in rats. In FASEB J on 2019 Nov by Mar n-Royo G, Rodr guez C, et al..PMID:31370681
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et al,A Preliminary Investigation of the Mechanisms Underlying the Effect of Berberine in Preventing High-Fat Diet-Induced Insulin
Resistance in Rats.In J Physiol Pharmacol on 2012 Oct by J-J Gu, F-Y Gao, et al..PMID:23211304
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et al,Metabolic inflammation exacerbates dopaminergic neuronal degeneration in response to acute MPTP challenge in type 2 diabetes mice.In Exp Neurol.On 2014 Jan by Wang L, Zhai YQ et al..PMID:24220636
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