产品详情
产品名称HSP27 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC
种属反应性Human
特异性The antibody detects endogenous level of total HSP27 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 80~84 (Q-L-S-S-G) derived from Human HSP27.
基因/蛋白名称HSP27
标记Unconjugated
别名28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; HSP 27; HSP25
数据库入口号Swiss-Prot: P04792
NCBI Protein: NP _001531.1
Uniprot
P04792
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from K562 and Hela cells using HSP27(Ab-82) Antibody #21240.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Ab-82) Antibody #21240(left) or the same antibody preincubated with blocking peptide(right).
Involved in stress resistance and actin organization.
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et al,Vasculo-protective Effect of BMS-309403 Is Independent of Its Specific Inhibition of Fatty Acid-Binding Protein 4.In Pflugers Arch on 2017 Sep by Yuta Okamura, Kosuke Otani ,et al..PMID: 28405802
, (2017),
PMID:
28405802
et al,Eukaryotic Elongation Factor 2 Kinase Controls Proliferation and Migration of Vascular Smooth Muscle Cells .In Acta Physiol (Oxf) on 2015 Feb by T Usui , R Nijima et al ..PMID:25069823
, (2015),
PMID:
25069823
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