产品详情
产品名称SAPK/JNK Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total SAPK/JNK protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.181~185 (M-M-T-P-Y) derived from Human SAPK/JNK.
基因/蛋白名称SAPK/JNK
标记Unconjugated
别名JNK2
数据库入口号Swiss-Prot: P45984/P53779
NCBI Protein: NP_001128516.1
Uniprot
P45984
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 46 54 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela and MCF cells using SAPK/JNK(Ab-183) Antibody #21241.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK(Ab-183) Antibody #21241(left) or the same antibody preincubated with blocking peptide(right).
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782
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et al,Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts.
, (2011),
PMID:
20709173
et al,Astragaloside IV Inhibits Isoprenaline鈥慽nduced Cardiac Fibrosis by Targeting the Reactive Oxygen Species/Mitogen鈥慳ctivated Protein Kinase Signaling Axis.In Mol Med Rep on 2017 Apr by Hongliang Dai ,Guizhi Jia ,et al..PMID:28260010
, (2017),
PMID:
28260010
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