产品详情
产品名称P38 MAPK Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total P38MAPK protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 180~184 (T-G-Y-V-A) derived from Human P38 MAPK.
基因/蛋白名称P38 MAPK
标记Unconjugated
别名MAPK2; MAPKAPK-2; MAPKAPK2
数据库入口号Swiss-Prot: Q16539
NCBI Protein: NP _001306.1
Uniprot
Q16539
计算分子量41kDa
实际分子量41kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000; IHC 1:50-1:200; IF 1:50-1:200;
Western blot analysis of extracts from 293 and Hela cells using P38 MAPK Antibody.
Western blot analysis of extracts from various cell lines using P38 MAPK Antibody.
Immunohistochemistry of paraffin-embedded Human placenta using P38 MAPK Antibody.
Immunohistochemistry of paraffin-embedded Rat kidney using P38 MAPK Antibody.
Immunohistochemistry analysis of paraffin-embedded Mouse liver using P38 MAPK Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Rat kidney using P38 MAPK Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human placenta using P38 MAPK Antibody.
Immunofluorescence analysis of NIH-3T3 cells using P38 MAPK Antibody.
Immunofluorescence analysis of L929 cells using P38 MAPK Antibody.
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
Ming Zheng, et al.(2005) The FASEB Journal. 19: 109-111
Bernt van den et al.(2001) Blink Immunology, 166: 582-587
Arshad Rahman, et al. (2004) Am J Physiol Lung Cell Mol Physiol 287: L1017-L1024
Osamu Yoshino, et al. (2003) Endocrinology & Metabolism Vol. 88: 2236-2241
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